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Foton-DOP / IGDR</acronym

Postdoctoral position in the area of optics and biology

starting before May 31st 2019 (24-month position)

Polarimetric orthogonally breaking imaging applied to the study of the dynamics of intracellular architecture (Polarbio)

Polarbio is a multidisciplinary project joining the expertises of the DOP team of the Institut FOTON , the IGDR and the imaging facility for life sciences of the Rennes 1 University. The objective of this project is to investigate the capacities of a new polarimetric sensing modality developed by the Institut FOTON for the imaging of biological samples. The collaboration between the members of the IGDR and Institut FOTON labs has allowed to set up a polarimetric confocal microscope based on the DSOB approach], which relies on the original illumination of samples by a dual-frequency dual-polarization laser light beam, and the detection of polarimetric signature through a single measurement involving demodulation of light at radiofrequencies. Initial “orthogonality-breaking” polarimetric images of living cells were recently acquired with this system.

First, the post-doctoral fellow will be in charge of establishing acquisition sequences able to assess specific polarimetric properties of the living tissues : diattenuation, depolarization, birefringence... These imaging modes will be set-up to allow acquisitions at high frame rates, which will be essential to characterize the dynamics of living tissues with optimal time-resolution.

Second, using the dual imaging capabilities of the setup which can be used simultaneously for fluorescence and polarimetric imaging, the post-doctoral fellow will image living cells tagged for specific structures such as the cytoskeleton or the chromatin. This will allow to precisely establish the polarimetric signatures of each of these different subcellular structures. Once this step completed, the post-doctoral fellow will analyze at high time resolution the dynamics of the chromatin architecture in untagged living samples. This structure, whose role in the maintenance of genome integrity has been studied by the IGDR team, is altered in many cancer cells and some specific alterations are already used for diagnostics in different types of cancer. Using polarimetric imaging, the post-doctoral fellow will characterize the chromatin architecture without the need for specific labeling, with the objective of identifying new characteristic structural parameters that could be used as diagnostic tools.


Please send the required documents by email to :
Julien FADE and Sébastien HUET with copy to UBL